Abstract
Summary The serum colloidal gold test is not acceptable as a substitute for the monkey serum neutralization test for poliomyelitis antibody. The complement fixation test employing antigens of monkey poliomyelitic spinal cord gave completely negative reactions with the sera of human beings containing antiviral antibody. The sera of normal and poliomyelitic immune monkeys gave completely negative Wassermann reactions. The sera of normal monkeys gave completely negative complement fixation reactions with antigens of monkey poliomyelitic spinal cord. Some of the sera of monkeys with poliomyelitis as well as some immunized with poliomyelitis vaccine or previously inoculated intracerebrally with neutralized serum-virus mixtures gave positive complement fixation reactions. All of the human and monkey sera employed in the complement fixation tests gave completely negative precipitation reactions with an antigen of monkey poliomyelitic spinal cord.
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