Terminal field specificity of forebrain efferent axons to brainstem gustatory nuclei

Abstract

Rostral forebrain structures like the gustatory cortex (GC), bed nucleus of the stria terminalis (BNST), central nucleus of the amygdala (CeA), and lateral hypothalamus (LH) send projections to the nucleus of solitary tract (NST) and the parabrachial nucleus (PBN) that modulate taste-elicited responses. However, the proportion of forebrain-induced excitatory and inhibitory effects often differs when taste cell recording changes from the NST to the PBN. The present study investigated whether this descending influence originates from a shared or distinct population of forebrain neurons. Under electrophysiological guidance, the retrograde tracers fast blue (FB) and fluorogold (FG) or green (GFB) and red (RFB) fluorescent latex microbeads were injected iontophoretically or by pressure pulses (10 ms at 20 psi) into the taste-responsive regions of the NST and the ipsilateral PBN in six rats. Seven days later, the animals were euthanized and tissue sections containing the LH, CeA, BNST, and GC were processed for co-localization of FB and FG or GFB and RFB. The results showed that the CeA is the major source of input to the NST (82.3 ± 7.6 cells/section) and the PBN (76.7 ± 11.5), compared to the BNST (31.8 ± 4.5; 37.0 ± 4.8), the LH (35.0 ± 5.4; 33.6 ± 5.7), and the GC (27.5 ± 4.0; 29.0 ± 4.6). Of the total number of retrogradely labeled cells, the incidence of tracer co-localization was 17 ± 3% in the GC, 17 ± 2% in the CeA, 15 ± 3% in the BNST and 16 ± 1% in the LH. Thus, irrespective of forebrain source the majority of descending input to the gustatory NST and PBN originates from distinct neuronal populations. This arrangement provides an anatomical substrate for differential modulation of taste processing in the first and second central relays of the ascending gustatory system.