Ten new glycosides, carissaedulosides A–J from the root barks of Carissa edulis and their cytotoxicities

Abstract

Ten previously undescribed glycosides, carissaedulosides A–J (1–10) referring to six apiosylated phenylpropanoids (1–6), one coumarin-secoiridoid hybrid (7), and three furofuran lignans (8–10) were isolated from the root barks of Carissa edulis, together with 13 known analogues (11–23). Their structures were elucidated by spectroscopic analysis, ECD computational methods, and chemical derivations for configurations of sugar moieties. The new lignan bisdesmoside, 10, exhibited significant cytotoxicity against A549 (IC50 = 3.87 ± 0.03 μM) and MCF-7 (IC50 = 9.231 ± 0.290 μM) cell lines, while the known lignan monodesmoside, 12, showed impressive cytotoxic efficacy (IC50 = 5.68 ± 0.180 μM) against only MCF-7 cell line. It is noted that a known cardenolide, 11, displayed strong cytotoxic potency against HL-60, A549, MCF-7 and SW480 cell lines with IC50 values ranging from 0.023 to 0.137 μM. Moreover, compound 11 induced dose-dependent apoptosis on SW480 cell, but not explicit dose-dependent apoptosis on HL-60 cells.