Abstract
Equilibrium and kinetic measurements of the thermal denaturation of tRNA1Val and tRNA2Tyr from Escherichia coli have been obtained by absorption melting curves and temperature‐jump experiments. Besides a very fast effect attributed to changes in stacking equilibria, several relaxation times in the millisecond range are observed at 260 nm. The incomplete separation of the different relaxation times suggests a complex process, in agreement with the equilibrium data. At 335 nm, where the absorption is due only to 4‐thiouridine, the melting profile is monophasic and for both tRNAs only one relaxation time is observed in the millisecond range. The thermodynamic parameters derived from the results obtained at this wavelength indicate a cooperative melting of short double helical sequences. The results are discussed on the basis of the clover‐leaf model.
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