Abstract
AIM: To determine telomerase activity of exfoliated cells in tuberculous peritonitis and malignant ascites, and study the diagnostic value of telomerase activity in differentiating tuberculous peritonitis from malignant ascites. METHODS: TRAP-PCR-ELISA and TRAP-PCR-silver staining were employed to determine telomerase activity in 18 specimens of malignant ascites and 13 specimens of tuberculous peritonitis. Telomerase activities in tuberculous peritonitis and malignant ascites were analysed. RESULTS: Telomerase activity in malignant ascites (0.387±0.023) was significantly higher than that in tuberculous peritonitis (0.023±0.004, P <0.01). The positive rate of telomerase activity in malignant ascites was significantly higher than that in tuberculous peritonitis, 88.9 % (16/18) vs 7.7 % (1/13), P <0.01. The sensitivity, specificity and accuracy of determination of telomerase activity in diagnosis of malignant ascites were 88.9 %, 92.3 %, and 90.3 %, respectively. CONCLUSION: Telomerase activity is positive in malignant ascites and may serve as a useful indicator for differentiating between tuberculous peritonitis and malignant ascites.
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