Abstract
This report describes for the first time a novel anionic background current (IAB) identified in guinea-pig isolated ventricular myocytes. It also shows that IAB has both novel and differential pharmacology from other (cardiac) chloride currents. Using the whole-cell patch-clamp technique and external anion substitution, IAB was found to be outwardly rectifying and highly permeable to NO−3, with a relative permeability sequence of NO−3 > I− > Cl−. IAB was not blocked by 50 μM DIDS, by hypertonic external solution, or by the nonselective protein kinase inhibitor H7-DHC. Exposure to the pyrethroid agent tefluthrin (10 μM) increased the current density of IAB significantly at positive voltages (P < 0.05), but had no significant effect on other cardiac chloride currents. We conclude that IAB possesses a distinct pharmacology and does not fall into the three major classes of cardiac chloride conductance commonly reported.
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