Abstract
The aim of this study was to determine the technological and safety properties of coagulase-negative staphylococci (CNS) with high protease activity isolated from traditional Chinese fermented sausages in 10 different regions. According to the criteria of meat starter cultures, eight strains were obtained from 76 Gram-positive and catalase-positive isolates of CNS. These strains could hydrolyze both sarcoplasmic and myofibrillar proteins, tolerate 10% of salts and 150 mg/kg nitrites, possessed good nitrate reductase activity and lipolytic activity, and exhibited non-decarboxylase activity. Furthermore, all the eight strains exhibited antibiotic susceptibilities and negative hemolytic activity, DNase activity, and biofilm formation ability. Whole genome sequencing demonstrated that there were no pathogenic genes in the eight CNS strains, including 18 enterotoxin genes, panton-valentine leukocidin, toxic shock syndrome toxin-1, exfoliative toxin genes, and biofilm genes. Based on the results of API and 16S rRNA sequence analysis, seven of the eight CNS strains were identified as Staphylococcus simulans and the remaining one was S. saprophyticus.
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