Abstract
Three strains of a d-xylose-fermenting yeast species were isolated from the host beetle Dorcus titanus collected from two different localities in Henan Province, Central China. These strains formed two hat-shaped ascospores in conjugated and deliquescent asci. Multilocus phylogenetic analysis that included the nearly complete small subunit (SSU), the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) rDNAs, as well as RNA polymerase II largest subunit (RPB1) gene demonstrated that these strains represent a novel yeast species belonging to the genus Scheffersomyces. The phylogenetic analysis based on the nucleotide sequences of the xylose reductase (XYL1) gene supported the view that the new strains could be grouped as a unique species. Although this new species is highly similar to Scheffersomyces stipitis-like yeasts in terms of nrDNA sequences and morphological and physiological characteristics, the species can be clearly differentiated from its close relatives on the basis of the sequences of XYL1 and RPB1. Therefore, a novel yeast species, Scheffersomyces titanus sp. nov., is proposed to accommodate these strains. The type strain is NYNU 14712T (CICC 33061T = CBS 13926T).
Highlights
Three strains of a d-xylose-fermenting yeast species were isolated from the host beetle Dorcus titanus collected from two different localities in Henan Province, Central China
The sequences of nuclear rDNAs, including small subunit (SSU), internal transcribed spacer (ITS) and large subunit (LSU), of the three strains of the new species were identical. This new species is closely related to the d-xylose-fermenting Scheffersomyces species, especially to S. stipitis and its close relatives, including S. henanensis, S. illinoinensis and S. segobiensis (Figs 1 and 2)
The new d-xylose-fermenting yeast species S. titanus was described and illustrated based on morphological and molecular characters. This new species is highly similar to S. stipitis-like yeasts in nrDNA sequences, as well as morphology and physiological characteristics (Table 1), the species can be clearly differentiated from its close relatives, S. henanensis, S. illinoinensis, S. segobiensis and S. stipitis, by the sequences of XYL1 and RPB1 (Figs 1 and 2)
Summary
Three strains of a d-xylose-fermenting yeast species were isolated from the host beetle Dorcus titanus collected from two different localities in Henan Province, Central China. The genus was later expanded by the inclusion of seven related Candida species as new combinations and by three novel species, namely, S. illinoinensis, S. quercinus and S. virginianus, which have been isolated from rotten wood[3]. This expansion resulted from a detailed multilocus phylogenetic analysis that included the traditional SSU and LSU markers, the orthologous RPB1 and the recently proposed ITS barcoding region for fungi[3,4]. During an investigation of the yeast community associated with insects obtained from central China, we isolated three d-xylose-fermenting yeasts whose physiological traits and ascospore morphology typically resembled www.nature.com/scientificreports/ We describe this new species as S. titanus sp. nov
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