Abstract
The purpose of the present study is to clarify the inhibitory effect of taurine on the elevation of cytoplasmic Ca2+ concentration ([Ca2+]i) in activated platelets. Using aequorin-loaded platelets activated with 1μM A23187 in normal subjects, [Ca2+]i was measured by a Platelet Ionized Calcium Aggregometer (Chrono-log). The elevation of [Ca2+]i in platelets activated with A23187 was significantly inhibited by the incubation with 1mM taurine for 10min. In order to elucidate the mechanism of this inhibitory effect of taurine, the aequorin-loaded platelets were pretreated with 5×10-5M aspirin (ASA) and 10-4M dibutyryl c-AMP (dbcAMP) before the incubation with 1mM taurine. The pretreatment with both of ASA and dbcAMP significantly inhibited [Ca2+]i in platelet activated with A23187 significantly. The elevation of [Ca2+]i in ASA-pretreated platelet activated with A23187 was further inhibited by the incubation with taurine, but in dbcAMP-pretreated platelet was not. These results suggest the possibility that the inhibitory mechanism of taurine on the elevation of [Ca2+]i in platelets activated with A23187 is not related to arachidonic metabolism. But it remains to be investigated wether the inhibition of taurine to platelet activation with A23187 is due to c-AMP-related mechanism or no related one.
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