Tailorable hydrogel of gelatin with silk fibroin and its activation/crosslinking for enhanced proliferation of fibroblast cells

Abstract

Gelatin based hydrogel (Gel) possess remarkable cytocompatibility profile rendering it appropriate for tissue engineering applications. Herein, the questionable mechanical property of Gel was tuned by tailoring with different loading concentrations of silk fibroin (SF). The as tailored matrix was reconnoitred for its physico-mechanical, chemical and biological properties in order to investigate the effect of SF loading. Ethanol treatment lead to enhance β-sheet formation of silk and subsequently, carbodiimide coupling was deployed to covalently crosslink the matrix. Substantial increase in cohesive energy with amplifying concentration of SF in the Gel matrix. As evidenced by Raman spectroscopy, right shift of the Amide I peak and stretching of COO− confirms activation of fibroin moiety along with crosslinking of gelatin, respectively. Moreover, with addition of SF, surface properties were tuned to attain maximum cell adhesion and proliferation. Further, MTT assay corroborated the same with definite increase in mitochondrial activities of L929 fibroblast cells for SF containing matrix as compared to its bare counterfeit while enhanced proliferation was confirmed by Rhodamine-DAPI staining. The alteration in mechanical and textural tribology of SF tailored Gel matrix certainly portrayed improved stability along with excellent cytocompatibility thus making it a plausible alternative in regenerative medicine.