Systematic toxicological analysis of the effect of salinity on the physiological stress induced by triphenyltin in Nile tilapia

Abstract

Triphenyltin (TPT), a synthetic chemical, is prevalent in complex salinity areas, including estuaries and coastal regions. However, current studies on the toxicological effects of TPT relevant to the environment at different salinities are limited. In the study, biochemical, histological, and transcriptional analyses of TPT and salinity alone, or in combination, was performed on the Nile tilapia (Oreochromis niloticus) liver. Nile tilapia exhibited weakened antioxidant defenses and liver damage. Transcriptomic analysis revealed that TPT exposure primarily affected lipid metabolism and immunity; salinity exposure alone particularly affected carbohydrate metabolism; combined exposure primarily immune- and metabolic-related signaling pathways. In addition, the single exposure to TPT or salinity induced inflammatory responses by up-regulating the expression of pro-inflammatory cytokines, whereas combined exposure suppressed inflammation by down-regulating pro-inflammatory cytokine levels. These findings are beneficial to understand the negative effects of TPT exposure in Nile tilapia in the broad salinity zones and its potential defense mechanisms.