Abstract
One of the most important compounds that exhibit a wide range of biological activities with especially strong antioxidant action are plant polyphenols. In the course of the experiment, the dose-dependent effects of polyphenols-rich extracts isolated from the Lamiaceae family Kazakhstani plants were studied on the processes of lipid peroxidation and on the degree of erythrocytes hemolysis. The activity of aqueous-ethanolic extracts from dried parts of plants, such as Origanum vulgare, Ziziphora bungeana, Dracocephalum integrifolium, Mentha piperita, Leonurus turkestanicus, Thymus serpyllum, and Salvia officinalis, was studied in a Wistar rat model. Lipid peroxidation (LPO) in liver microsomes was assessed by measuring malondialdehyde content in the form of thiobarbituric acid-reacting substances (TBARS). Estimation of osmotic resistance of isolated erythrocytes was evaluated based on hemoglobin absorbance. The amount of total phenolics in the extracts was measured using the Folin-Ciocalteu reagent method. Based on the results, Thymus serpyllum extract exhibited a significantly higher antioxidant activity (IC50 = 3.3 ± 0.7) compared to other plant extracts. Accordingly, among the extracts studied, those from Salvia officinalis, Thymus serpyllum, and Origanum vulgare show the most pronounced membrane-stabilizing activity. Antioxidant and antihemolytic properties of green tea and Origanum vulgare extract mixtures were similar to that of each individual plant extract. Similar results were obtained when the green tea extract was mixed with Mentha piperita, Ziziphora bungeana, and Dracocephalum integrifolium extracts, indicating no discernible synergistic interaction.
Highlights
We have previously shown that all used plant extracts exhibit antioxidant properties, protecting membranes from lipid peroxidation
When compared with black tea, green tea has more beneficial effects on living organisms. Both types of tea exert dose-dependent antioxidant effects on membranes of hepatocytes in vitro [48]. Comparison of their antioxidant activities showed that the green tea properties are stronger than those of the black tea
It has been shown that extracts of oregano, thyme, and sage have a protective effect on both erythrocyte membranes and microsomal fractions of hepatocytes
Summary
Plants have a large number of important biochemical compounds that are beneficial to cells, tissues, and the whole organism. Biological activities of such compounds depend on their chemical structure. Polyphenols and flavonoids constitute one of the major groups of secondary metabolites that occur in staple food plants, where they perform a wide array of biological functions [1,2]. Despite the differences in their chemical structure and composition, flavonoids are strong natural antioxidants, which neutralize free radicals, prevent from damaging cells and tissues, and prevent occurrence of pathological processes [3]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
