Abstract
Two anti-inflammatory peptides corresponding to a high amino acid similarity region between lipocortins were synthesized and tested on their ability to inhibit porcine pancreatic phospholipase A 2. Kinetic assays using monomeric and aggregated phospholipids did not reveal any phospholipase A 2 inhibitory activity. The peptides did not inhibit phospholipase A 2 activity on monolayers of negatively charged substrate and did not prevent phospholipase A 2 action on mixed micelles of 1-stearoyl-2-arachidonoyl- sn-glycero-3-phosphocholine and sodiumdeoxycholate. Ultraviolet difference spectroscopy did not show binding of the peptides to phospholipase A 2. Therefore we conclude that these anti-inflammatory peptides do not inhibit pancreatic phospholipase A 2 in vitro, in contrast to the results recently published [(1988) Nature 335,726–730].
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