Abstract
Cadmium selenide (CdSe) nanoparticles (NPs) were first synthesized in the cavity of recombinant cage-shaped protein from marine pennate diatoms. Our constructed recombinant apoferritin (FerA-dCys) from the diatoms revealed that the removal of cysteine residues on the surface of the recombinant apoferritin enhanced the CdSe NPs synthesis in the apoferritin cavity. The CdSe NPs were synthesized in the FerA-dCys with a very low concentration of cadmium ions (0.02mM). The core formation ratio of CdSe NPs showed more than 20%. The average diameter of synthesized CdSe NPs was 7nm with a small size distribution. The synthesized NPs were analyzed by energy-dispersive X-ray spectroscopy (EDX) and high-resolution transmission electron microscopy (HR-TEM), and it was confirmed that the synthesized NPs in the FerA-dCys were consistent with Cd and Se ions.
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