Abstract
In this study, a novel biocompatible hydroxyapatite (HA) was synthesized by using chitosan oligosaccharide (COS) as a template. These HA samples were studied by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and transmission electron microscopy (TEM). The biocompatibility of HA samples was evaluated via cell viability, cell morphology and alkaline phosphatase staining of MG-63 cell lines. The results show that HA synthesized in the presence of COS was favorable to proliferation and osteogenic differentiation of MG-63 cells. These hydroxyapatites are potentially attractive biomaterials for bone tissue engineering applications.
Highlights
Various biomaterials have been developed for bone tissue engineering applications such as bone graft substitutes, bone repair, biodegradable bone scaffold and bone drug carriers [1,2,3,4]
With stricter biocompatibility requirements for materials used in clinic application, biomimetic synthesis of hydroxyapatites with improved biocompatibility is a promising strategy for the clinic application of hydroxyapatites for bone tissue engineering [5,6,7]
Wang et al [11] demonstrated that the nucleation, growth, orientation and structure of bone apatite crystals was predominantly controlled by collagen matrix
Summary
Various biomaterials have been developed for bone tissue engineering applications such as bone graft substitutes, bone repair, biodegradable bone scaffold and bone drug carriers [1,2,3,4]. Biomolecules including polysaccharides [6,9,10], collagens [11,12], gelatin [13,14] and peptides [15,16,17] have attracted extensive attention in regulating HA nucleation and growth during the mineralization process. Wang et al [11] demonstrated that the nucleation, growth, orientation and structure of bone apatite crystals was predominantly controlled by collagen matrix. Wise et al [18] recently suggested that polysaccharides, not proteins, predominantly form an organic–mineral interface. The functional groups of polysaccharides can chelate Ca2+ ions and form hydrogen bonds with protonated PO43 ́ and H2O on the surface of the bone apatites [19]
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