Abstract
The novel water-soluble conjugated polyelectrolyte containing a new kind of backbone poly[(4,4-bis(3ʹ-N,N,N-trimethylammonium) propyl) cyclopentadithiophene dichloride] (PBTPC-Cl) is synthesized and characterized. The PBTPC-Cl is readily soluble in polar solvents and is applied as a sensitive and selective fluorescent probe for heparin detection in aqueous solution. Upon adding heparin into the aqueous solution of polymer, PBTPC-Cl could bind heparin via electrostatic interactions and results in the quenching of emission peaks. Quantitative analysis of heparin shows high selectivity and good linear relationship with a detection limit to 0.05 μM in HEPES aqueous buffer.
Highlights
Within the past few years, conjugated polyelectrolyte (CPE) that contains a conjugated polymer backbone and charged groups has attracted considerable attention.[1]
The CPDT was chosen as starting material and the polymer was synthesized via bromination, amination, and the polymer was synthesized by oxidative polymerization in chloroform using FeCl3 as the oxidant.[25,26]
The novel water-soluble cationic PBTPC-Cl based on the PCPDT backbone was successfully synthesized and applied to heparin detection
Summary
Within the past few years, conjugated polyelectrolyte (CPE) that contains a conjugated polymer backbone and charged (anionic or cationic) groups has attracted considerable attention.[1]. Fluorescent assays have been proved to be an efficient and sensitive method for heparin detection or quantification This kind of method uses water-soluble conjugated polymers containing cationic groups and has been successfully applied in multicolor detection assay for trace heparin in aqueous solution via the electrostatic interactions.[22,23] The proposed polymer is water-soluble and has the potential applications in biological environment. To extend the potential applications of the water-soluble PCPDT, we report the design and synthesis of new watersoluble conjugated polymer PBTPC-Cl. The proposed polymer has good water solubility and efficient signal for heparin quantification in the range of 0.05 to 20 μM
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