Abstract
[ Methylene- 14C]FD & C Blue No. 1 was synthesized in eight steps from barium [ 14C]carbonate. Female Sprague-Dawley rats were given a single dose (0·27 mg; 1·74 μCi) of the 14C-labelled colouring by gavage. In bile-duct ligated rats, intestinal absorption of FD & C Blue No.1 (estimated from urinary 14C excretion, expired 14CO 2 and residual radioactivity in internal organs and tissues 96 hr after oral administration) averaged 2·05% of the dose. mean faecal excretion was 97·28% and the total recovery of administered radioactivity was 99·38%. Intestinal absorption [ 14C]FD & C Blue No. 1 in intact rats averaged only 0·27% (91·% recovery), while biliary excretion in bile-duct cannulated animals averaged 1·32% of the dose. Thin-layer chromatography of urine and bile samples revealed that about 95% of excreted radioactivity was unaltered FD & C Blue No. 1 and that about 5% was an unidentified metabolite or degradation product of FD & C Blue No. 1. The results show that Fd & C Blue No.1 is poorly absorbed from the gastro-intestinal tract, and undergoes subsequent rapid and complete biliary excretion.
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