Abstract
A thiazolidine-2-thione mid-functionalized chain transfer agent (CTA) was synthesized and used as a reversible addition−fragmentation chain transfer (RAFT) polymerization agent to prepare poly(N-(2-hydroxypropyl)methacrylamide) (polyHPMA) with mid-chain thiazolidine-2-thione functionality. The synthesized polymers were fully analyzed by 1H NMR and GPC, confirming well-defined structures (predesigned molecular weights, narrow polydispersities, and high functionalization efficiencies). A subsequent hydrolysis/analysis of the polymers was performed to verify their mid-functional structures. These mid-functionalized polymers were then incubated with a model protein (lysozyme) to generate branched polymer−protein bioconjugates. The bioactivity of the branched polymer−protein conjugate was tested and compared to similar molecular weight linear polyHPMA−protein bioconjugate; the branched polymer−protein conjugate remained much more protein activity, indicating the mid-chain-functional polyHPMA was more selective...
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