Abstract
Generation of well-defined potential metallotherapeutics for cancer treatment, one of the most population-threatening diseases, is challenging and an active area of modern research in view of their unique properties and thus multiple possible pathways of action in cells. Specifically, Schiff base ligands were recognized as very promising building blocks for the construction of stable and active complexes of numerous geometries and topologies. Incorporation of Ag(I) ions allows for the formation of flat complexes with potential unoccupied coordination sites, thus giving rise to specific interactions between the metallotherapeutic and biomolecule of interest. Herein, we present the design, synthesis and characterization of new Schiff base ligand L and its Ag(I) bimetallic complex [Ag2L2]2+ with two planar moieties formed around the metal ions and connected through cyclohexane rings, confirmed by X-ray measurements. The compounds were described in context of their potential use as anticancer drugs through DNA and BSA binding pathways by several spectroscopic methods (CD, UV-Vis, fluorescence). We revealed that both, L and [Ag2L2]2+, interact with similar affinity with CT-DNA (Kb~106 M−1), while they differ in the type and strength of interactions with the model albumin–BSA. [Ag2L2]2+ binds BSA in both a dynamic and static manner with the Ksv = 8.8 × 104 M−1 in the Trp-134 and Trp-213 sites, whereas L interacts with BSA only dynamically (KSV = 2.4 × 104 M−1). This found further confirmation in the CD studies which revealed a reduction in α-helix content in the albumin of 16% in presence of [Ag2L2]2+.
Highlights
The antimicrobial properties of silver ions have been known since ancient times [1].They are toxic to certain types of bacteria, viruses and fungi, their toxicity to mammalian cells is low, enabling the use of silver compounds in cancer therapies [2]
The N4 -donor Schiff base ligand L is a product of condensation of one equiv. of 1,4-trans-diaminocyclohexane and two equiv. of 2-thiazolecarboxaldehyde
The possible binding mechanisms to relevant biomolecules, DNA and Bovine Serum Albumin (BSA), of the new Schiff base ligand L and its bimetallic complex [Ag2L2](PF6)2 were investigated by spectroscopic methods
Summary
The antimicrobial properties of silver ions have been known since ancient times [1] They are toxic to certain types of bacteria, viruses and fungi, their toxicity to mammalian cells is low, enabling the use of silver compounds in cancer therapies [2]. This metal has been found in trace amounts in 29 human tissues, but the importance of silver in the functioning of the body has never been established. The disadvantage of cisplatin and its derivatives (carboplatin and oxaliplatin)
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