Abstract
Nano-encapsulation of catechin (CAT) with beta-cyclodextrin (BCD) at 1:1 ratio resulted in the production of nano-encapsulated powder (BCD+CAT) by molecular inclusion method. The interactions between CAT and BCD were analyzed by differential scanning calorimeter (DSC), Raman laser spectroscopy (RLS), scanning electron microscopy (SEM) and X-ray diffractometer (XRD). The results obtained strongly confirmed supra molecular complex formation. DSC thermograph obtained for nano-encapsulated powder resulted in the appearance of new endothermic melting peak at 250°C with complete disappearance of peaks at 100, 150 and 200°C present in catechin and beta-cyclodextrin indicating the inclusion complex formation which was also confirmed by spectral characterization (RLS). Particles ranging from 67 to 470nm were obtained by SEM micrographs. Structural characterization by XRD at 2θ angle from 0° to 80° for nano-encapsulated powder indicated change of state from crystalline to amorphous phase with average particle size of 518.78nm.
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