Abstract
An amperometric glucose biosensor was prepared successfully based on a conducting polymer, poly(4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine) (poly(SNS-NH2)) and a flexible hydrophilic polyphosphazene polymer, poly[(methoxyethoxy)ethoxy-co-3-formylphenoxy]phosphazene (PPA). Poly(SNS-NH2) was electrochemically polymerized on a graphite electrode to achieve a conducting immobilization matrix to improve the enzyme immobilization on the transducer surface. Moreover, to strengthen the immobilization, a polyphosphazene derivate bearing functional aldehyde group was designed, synthesized and used in the immobilization of glucose oxidase. Not only the amino groups in the structure of poly(SNS-NH2), but also the aldehyde groups in PPA were contributed to the covalent immobilization as well as the entrapping the biomolecules in PPA network during the immobilization process. This afforded an effective and long-life analysis of glucose. Amperometric measurements were conducted at −0.7V vs. Ag/AgCl in 50mM sodium acetate buffer at pH 4.5. KMapp (0.677mM), Imax (20.91μA), LOD (1.3μM) values were determined. Moreover, biosensor showed an extremely high sensitivity as 237.1μAmM−1cm−2 owing to the newly synthesized and combined highly flexible hydrophilic polymeric immobilization matrix. Finally, the proposed biosensor was successfully applied for determination of glucose content in several beverages, successfully.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
