Synthesis and biological evaluation of some pyrazolylpyrazolines as anti-inflammatory–antimicrobial agents

Abstract

A new series of pyrazolylpyrazolines ( 5a– k) was synthesized by the reaction of appropriate chalcones ( 3a– k) with 4-hydrazinobenzenesulfonamide hydrochloride ( 4) in ethanol. All the newly synthesized target compounds ( 5a– k) were screened for their anti-inflammatory activity using carrageenan-induced rat paw edema assay. Compounds 5g and 5j showed pronounced anti-inflammatory activity comparable to the reference standard nimesulide, whereas, compounds 5b, 5d and 5h displayed good anti-inflammatory activity. Additionally, the synthesized compounds were evaluated for their in vitro antimicrobial activity against two Gram-positive bacteria and two Gram-negative bacteria. Four compounds 5c, 5h– 5j showed good broad spectrum activity against all the tested Gram-positive and Gram-negative bacterial strains. Compound 5j could be identified as the most biologically active member within this study with an interesting dual anti-inflammatory and antibacterial profile.