Abstract
Purpose: Synovial fluid (SF) reflects the biological milieu of the joint and is a potentially important source of biomarkers in osteoarthritis (OA). OA joints usually do not offer enough SF volume for analysis of multiple markers in a single sample by conventional enzyme-linked immunosorbent assays (ELISA). Newer platforms i.e. Luminex and Meso Scale Discovery (MSD) enable multiplex analysis in small (≤50μl) volumes. However, SF analysis is challenging due to its complex matrix and viscous non-Newtonian nature.
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