Synergy of an IgH promoter-enhancer-driven c-myc/v-Ha-ras retrovirus and pristane in the induction of murine plasmacytomas.

Abstract

Intraperitoneal injection of BALBc/An mice with a single 0.5 ml dose of the mineral oil pristane leads to the appearance of malignant Ig-secreting (usually IgA) plasma cells in 25% of mice after minimal latent periods of 120 days and mean latent periods between 210–220 days (Potter and Wax 1983). Over 95% of these plasmacytomas (PCTs) contain rcpt(12;15) or rcpt(6;15) chromosomal translocations in which the c-myc gene on chromosome 15 is juxtaposed with immunoglobulin heavy or light chain loci on chromosomes 12 and 6 respectively. These structural alterations disrupt the normal regulation of the c-myc gene which is thought to be causally related to the development of malignancy (reviewed in Potter 1984). Indeed infection of pristane primed mice with wild-type Moloney (Mo-MuLV) helper virus and the replication defective J-3 retrovirus, which contains a partially deleted raf oncogene and a hybrid MH2/MC29 avian v-myc oncogene induced shortened latency plasmacytomas which lacked c-myc translocations (Potter et. al. 1987). On the other hand experiments which used murine retroviruses harboring an LTR driven murine c-myc gene only induced myeloid malignancies in pristane primed mice (Wolff et. al. 1986).KeywordsHelper VirusLight Chain LocusRearrange Immunoglobulin Heavy Chain GeneMinimal Latent PeriodRearrange Immunoglobulin Heavy ChainThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.