Synergistic Osteogenic Potential of Human Mesenchymal Dental Pulp Stem Cells and Platelet-Rich Plasma on Repair of Anterior Maxillary Bone Defect

Abstract

Purpose: The aim of the present study was to evaluate synergistic osteogenic potential of human dental pulp mesenchymalstem cells (hDMSCs) and platelet-rich plasma (PRP) in enhancement of bone regeneration in anterior maxillary bone defects.Patients and methods: Eighteen patients (10 males and 8 females), were selected and divided equally into three groups. Group I included patients (4 male and 2 female ) having anterior maxillary cyst and impacted or supernumerary teeth in another place indicated for surgical removal for production of mesenchymal dental pulp stem cells, group II and III included patients (3 male and 3 female in each) having anterior maxillary cyst. The cyst was enucleated and the space left were filled by hDMSCs with PRP in group I. in group II the cavity was filled by PRP only. In group III the cystic cavity left with no filling material and considered as control group. Cone Beam Computed Tomography (CBCT) was performed preoperatively, one month and six months postoperatively for comparison of bone density intra group and inter groups. Results: Comparison of bone density between the three groups at one month post-operative period showed that group II recorded highest and significant bone density. At six months post-operative period the three groups showed significant increase in bone density However group I (hDMSCs with PRP) showed highest mean increase in bone density. Conclusions: hDMSCs can provide an osteogenic cell source for new bone formation and the PRP improves and retains their differentiation capacity due to possible synergisticosteogenic potential between PRP and stem cells.