Abstract
The present study was aimed to develop a rapid in vitro propagation procedure for Quercus serrata. Seeds, (1) without seed coat (scarified); (2) scarified seeds cut at different lengths from distal ends; and (3) with seed coat (un-scarified), were subjected to various treatments. The scarified and half cut seeds, inoculated on woody plant medium supplemented with N6benzylaminopurine (BAP: 8.88 µM) plus indole-3-acetic acid (IAA: 2.86 µM), produced significantly (p < 0.05) higher number of shoots within 9.50 ± 2.29 days. In vitro derived shoots rooted under two-step rooting procedure and exhibited 100% rooting upon application of indole-3-butyric acid (IBA: 100 μM) for 48 h. The survival rate of Q. serrata plantlets was 95% in the greenhouse. Thus, this “cut-seed method” can be used for the rapid regeneration of Q. serrata and can be replicated to the other threatened and high-value tree species for their conservation and mass multiplication.
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Schedule a callMore From: Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
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