Abstract
The level of rat hepatic ornithine decarboxylase (ODC) induced by repetitive administration of Co 2+ was determined by affinity labeling with [ 3H]difluoromethylornithine. Such a treatment with Co 2+ ion induced ODC level to a 10-fold greater extent than single dose of the metal ion or well-known inducers of the enzyme, such as thioacetamide or carbon tetrachloride. The half life of ODC activity induced by repetitive treatment with Co 2+ (95 min) was substantially increased to about 10-fold over the value obtained from the enzyme induced by single treatment with the metal ion (10 min). ODC activity induced by repetitive treatment with Co 2+ was separated into two peaks by DEAE-Sepharose column chromatography. The two independently collected fractions of ODC peaks exhibited different affinity for pyridoxal 5′-phosphate in vitro and sensitivity to cycloheximide in vivo.
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