Abstract
Combining multiple drugs or biologically active substances for wound healing could not only resist the formation of multidrug resistant pathogens, but also achieve better therapeutic effects. Herein, the hydrophobic fluoroquinolone antibiotic ciprofloxacin (CIP) and the hydrophilic broad-spectrum antibiotic tetracycline hydrochloride (TH) were introduced into the coaxial polycaprolactone/gelatin (PCL/GEL) nanofiber mat with CIP loaded into the PCL (core layer) and TH loaded into the GEL (shell layer), developing antibacterial wound dressing with the co-delivering of the two antibiotics (PCL-CIP/GEL-TH). The nanostructure, physical properties, drug release, antibacterial property, and in vitro cytotoxicity were investigated accordingly. The results revealed that the CIP shows a long-lasting release of five days, reaching the releasing rate of 80.71%, while the cumulative drug release of TH reached 83.51% with a rapid release behavior of 12 h. The in vitro antibacterial activity demonstrated that the coaxial nanofiber mesh possesses strong antibacterial activity against E. coli and S. aureus. In addition, the coaxial mats showed superior biocompatibility toward human skin fibroblast cells (hSFCs). This study indicates that the developed PCL-CIP/GEL-TH nanofiber membranes hold enormous potential as wound dressing materials.
Highlights
Published: 8 February 2022As a global public health problem, wound healing has always been the focus of clinical treatment [1]
The PCL loaded with CIP acts as the core layer, while the GEL loaded with tetracycline hydrochloride (TH) acts as the shell layer,and and dual drug-loaded nanofiber membranes were prepared by coas the shell layer, thethe dual drug-loaded nanofiber membranes were prepared by coaxial axial electrospinning
The inner and outer layers of fibers are respectively loaded with two antibiotics with different hydrophilicity and hydrophobicity, which are released in sequence
Summary
As a global public health problem, wound healing has always been the focus of clinical treatment [1]. The drugs in the inner and outer layers of the PCL/GEL nanofiber membrane are released at different stages of the wound healing with the quick release of TH for 12 h and the long-lasting release of CIP for 5 days, realizing the antibacterial synergy of drug-loaded dressings. This indicates that the nanofiber membrane can reduce the possibility of the formation of drug-resistant bacteria, but can meet the antibacterial needs in the wound healing process, promoting the wound healing. This research may provide valuable insight into the development of a dual drug delivery system to promote wound healing
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