Abstract
BackgroundIn recent decades neoadjuvant therapies such as combined chemotherapy and radiotherapy have been introduced for cancer management. Compared with monotherapy modalities, neoadjuvant therapy is associated with greater effectiveness while having minor side effects. Docetaxel is a chemotherapy agent for breast cancer treatment which can blocks the cell cycle at the G2/M phase which has shown special sensitivity to the ionizing radiation and hence causes cell death. To the best of our knowledge, there are currently no reports that explore the synergistic effects of Docetaxel and ionizing radiation on MCF-7 cancer cell death.MethodsWe divided cells into four different groups; control, cells which got in touch with Docetaxel, cells that with exposure to radiotherapy and cells which were influenced with combination of Docetaxel and radiotherapy. In vitro cell viability tests were done at different concentration of Docetaxel and different dose of radiation for 24, 48 and 72 h after the experiment.ResultsResults showed that the cytotoxicity was depending on the doses of radiation and Docetaxel. Radiation at 2 Gy dose was unable to produce significant effects neither in the radiation-only nor in the neoadjuvant therapy groups. However, the synergistic effects of neoadjuvant therapy were apparent at 4 and 6 Gy doses of radiation which could exert more significant cytotoxic effects on MCF-7 cells.ConclusionsStudy findings suggest that neoadjuvant therapy by using Docetaxel and 4 and 6 Gy ionizing radiation has synergistic effects on MCF-7 cell death and produces more significant results compared with monotherapy modalities.
Highlights
In recent decades neoadjuvant therapies such as combined chemotherapy and radiotherapy have been introduced for cancer management
Drugs which can impede the movements and activities of microtubules are used for inhibiting cell division and thereby, treating malignancies such as metastatic breast cancer [6]
We depict that Docetaxel-alone and the neoadjuvant therapy caused significantly higher MCF-7 cell death at 75, 100, and 200 μM doses of Docetaxel compared with the control group
Summary
In recent decades neoadjuvant therapies such as combined chemotherapy and radiotherapy have been introduced for cancer management. Docetaxel is a chemotherapy agent for breast cancer treatment which can blocks the cell cycle at the G2/M phase which has shown special sensitivity to the ionizing radiation and causes cell death. In this regard, one of the innovative neoadjuvant therapies is the combination of Docetaxel chemotherapy agent with radiotherapy. Drugs which can impede the movements and activities of microtubules are used for inhibiting cell division and thereby, treating malignancies such as metastatic breast cancer [6]. Taxaneanti-cancer agents such as Docetaxel and Paclitaxel (Taxol®) are anti-cancer drug which exerts their anti-cancer effects through intracellular damaging [7] They disturb the balance between the polymerization and depolymerization of tubulin dimmers through inhibiting either the polymerization of tubulin dimmers or the depolymerization of already formed dimmers [8]. When the amount of active ß-tubules is decreased, the production of tubulin dimmers is inhibited [9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
