Abstract
A novel synchronous fluorescence method is described for determination of phytic acid in food samples. It is based on the formation of a ternary complex between phytate, 1,10-phenanthroline (phen) and Fe3+. The synchronous fluorescence intensity of the solution was accordingly enhanced proportionally to the increased phytate concentration. Synchronous luminescence spectroscopy was adopted in the study, and the Δλ was set to 40 nm. The calibration graph is linear from 0.33 to 32 mg L−1 with a linear equation of I f = 8.770 + 2.980c (R 2 > 0.9994). The method was applied to determine phytate in food samples and the found concentrations of phytic acid in food were in the range of 4.62–24.08 mg g−1 with recoveries of 92.2%–98.3%. The control experiments were performed using UV-spectrophotometry method, and the results showed the method to be reliable.
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