Abstract
Biochemical studies of the sporulation process in mycelial fungi require a thallus in which the conidiation (production of conidiophores and conidia) will take place in a synchronous manner.A method was worked out which permits an approximate evaluation of 5 degrees of the synchronous sporulation of Penicillium digitatum. A suitable nutritional regime for the cultivation of the fungus as mycelial felts was found to promote synchronous sporulation. A defined medium containing a vitamin mixture lacking p-amino benzoic acid (PABA) gave satisfactory growth and good synchronization. Other methods such as starvation of the mycelium, cold shock, etc. had little effect.
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