Abstract
Synchronisation by hydroxyurea does not affect the sensitivity of CEM-C7 lymphoblasts to glucocorticoids.
Highlights
Studies of steroid-drug interactions in CEM-7 cells have revealed differences between some drugs which act in S-phase of the cell cycle
Our original hypothesis to explain the antagonism between prednisolone and the latter two drugs consisted of two main proposals: (i) MP
Allowed cells which had been partially synchronised in S-phase to be released, in the presence of hormone, into phases of the cell cycle which we presumed to be relatively more steroid sensitive
Summary
Studies of steroid-drug interactions in CEM-7 cells have revealed differences between some drugs which act in S-phase of the cell cycle. Ara-C cause an inhibition of DNA synthesis which is, to some extent, reversible and (ii) cells blocked in S-phase are protected from the lethal effect of steroid. Measurement of cellular DNA content by flow microfluorimetry (FMF) confirmed that Ara-C blocked CEM-C7 cells in S-phase, and that the block was reversible (Gledhill et al, 1983).
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