Abstract
GA20 oxidase (GA20ox) is an enzyme that regulates GA (Gibberellic acid) biosynthesis and is essential in maintaining the dynamic balance of gibberellin in plants. We identified the PavGA20ox-2 gene from sweet cherry. The expression level of PavGA20ox-2 increased from winter to spring and significantly decreased after GA4+7 treatment. Surprisingly, the expression level of PavGA20ox-2 in sweet cherry flower buds with transient infection of overexpression PavWRKY31 was significantly higher than that in the control group. Besides, PavWRKY31 is directly bound to the W-box motif in the promoter of PavGA20ox-2 to activate the transcriptional level of PavGA20ox-2. The plant height was also increased, and the flowering time was advanced when PavGA20ox-2 was overexpressed in Arabidopsis. Moreover, the seed germination rate of Arabidopsis thaliana was higher than that of the wild type. In conclusion, PavGA20ox-2 could promote plant growth, accelerate flowering time, and increase seed germination. Thus, PavGA20ox-2 is considered involved in regulating multiple processes of plant growth and development by affecting GA biosynthesis. Our results will contribute to a better understanding of the molecular mechanism of GA20ox-mediated fruit tree development.
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