Abstract
Anemia is a serious problem in the neonate. To investigate the pathophysiology of neonatal anemia, to assess responses to blood transfusions and therapy, and to comparatively evaluate various sources of RBC and methods for RBC storage, direct measurement of RCS would be useful.MethodsTo develop a method for simultaneous measurements of RCS, the density of biotin label on RBCs (BioRBC) was incrementally increased to produce 4 distinct BioRBC populations. Following reinfusion, blood was sampled over the next 4 mo, and the decline in BioRBCs was determined by flow cytometric enumeration.ResultsBioRBC at reagent concentrations < 81 μg/mL RBC produced superimposable linear disappearance curves (Fig) and normal survival (mean potential lifespan=117±5 d). BioRBC at 243 µg/mL disappeared rapidly and were not suitable for RCS.ConclusionRCS of distinct populations of infused RBCs in humans can be measured accurately using enumeration of BioRBC. RCS of different blood sources (e.g., placenta, donor, and autologous) can be simultaneously tracked. Supported by NIH P01 HL046925; Thrasher Research Foundation 02825‐3.
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