Abstract
Summary Combinations of 20% egg yolk with 1.5 to 3.0% solutions of glycine resulted in poor survival of spermatozoa. Inclusion of 10% glycerol resulted in sperm survival comparable to that obtained in 50% egg yolk-glycine extenders with or without glycerol. A 20% egg yolk extender containing 1.16% sodium citrate dihydrate, 0.75% glycine, and 1.0% glucose was evolved. Twenty ejaculates of semen stored in this extender averaged 41% motile spermatozoa after 12 days of storage at 5° C. Results at 5° C. were superior to those at 25° C. (P 0.05). The high sperm survival in both of these extenders at 5° C. indicated they might be useful in preserving the fertility of sperm stored for several days at this temperature.
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