Abstract
ABSTRACT The effect of different strategies of opening water flow on the survival and growth of Haliotis rufescens 9-d postlarvae was evaluated using a randomized block design with 3 replicates for 63 days. Larvae were settled (1.5 µM gamma-aminobutyric acid) in 2-L plastic vessels (total area, 701 cm2) and were subjected to the following treatments: static conditions, static conditions with aeration (NF + A), flow opened after 10 d, flow opened after 17 d, and flow opened after 24 d. The experiment was conducted at constant temperature (17.5 ± 0.5°C) and light intensity (∼750 lux). Water was changed daily in static treatments, and flow treatments received filtered seawater at ∼60 mL/min. Postlarvae were fed the benthic diatom Navicula incerta per standardized protocols. Every week, all organisms in each experimental unit were counted to estimate survival, and samples of 15 postlarvae were recorded under an inverted microscope and measured using digital image analysis. At the end of the experiment, greate...
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