Abstract
In the present study surfactant mediated extraction has been investigated for the extraction of antioxidants from clove flower buds (Syzygium aromaticum). The advantage of this technique is the replacement of organic solvents or other toxic substances by surfactants that could be part of a formulation. The influence of the Hydrophilic Lipophylic Balance (HLB) on extraction was examined in the range of 4.3 to 15 by the mixture of two commonly used surfactants in pharmaceutical and food applications (Tween 80 and Span 80). The effect of the surfactant concentration (2, 5, and 10%) was also evaluated. Results showed that higher HLB values improved eugenol and polyphenols extraction, which could be correlated with the in vitro antioxidant activity. Surfactant concentration of 5% (w/w) showed to be more effective than 2% and there were no significant differences between the results obtained at concentrations of 5 and 10%. The effect of the pH was also investigated on Tween 80 solutions and results showed that increases on the pH value improved the eugenol extraction.
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