SURFACE FUNCTIONALIZED EMULSION FOR SELECTIVE DRUGS TRANSPORT

Abstract

This paper presents the results of functionalization of surfaces of double emulsions drops for selective drug transport and selection of antibodies for targeting brain tumor cells - glioblastoma multiforme (GBM). The functionalization involved physical adsorption of antibodies: anti-CD15 (Hodgkin's disease marker) on oil drops of the emulsion. The interactions between molecules of antibodies and compounds of the oil drops were measured using isothermal titration calorimetry (ITC). Double emulsions were prepared by a one-step method, in a helical flow contactor. The ITC analysis showed that antibodies interacted with emulsion compounds. The change in the heat rates reflecting molecular interactions proved that proteins adhered to oil drops interfaces. Additionally, surface proteins (antibodies) were identified and analyzed for targeting specific GBM cell lines: U87MG, LN229, T98G. The multiple emulsions were created under conditions: the size of the annular gap between the coaxial cylinders was 1.5 mm, the rotational frequency of the inner cylinder was 2162 rpm, the volumetric flow rates of internal water phase to membrane oil phase 1:1 and membrane oil phase to external water phase 1:15. The method of double emulsions preparation has been described more precisely in our previous papers. The sizes of the internal phase drops and the membrane phase drops were determined based on analyzing images captured during microscopic observation of the double emulsion samples.