Supramolecular fluorescent probe based on acyclic cucurbituril for detection of cancer Labels in human urine.

Abstract

Spermine (Spm) and spermidine (Spmd) are considered as potential biomarker for early diagnosis of human cancer. Herein, a novel acyclic cucurbituril derivative (UL-ACB) was firstly designed and synthesized, which fluoresces at 460nm after excitation at 365nm. UL-ACB is rich in oxygen atoms which are capable of forming coordinate bonds with copper (Cu2+) that cause quenching of UL-ACB fluorescence. Moreover, the addition of biological endogenous substances Spm and Spmd can turn on fluorescence of UL-ACB. Interestingly, the probe showed a remarkable detection efficiency for Spm and Spmd in human urine (the detection limits of Spm and Spmd were 0.156μM and 0.762μM, and the linear ranges are 0.156∼43.06μM and 0.762∼29.10μM), which completely covered the early diagnosis of urinary Spm (1∼10μM) and urine Spmd (1∼20μM) required concentration range in cancer patients. The probe for Spm and Spmd is simple, time-saving and selective, which may provide a new promising strategy for early cancer diagnosis.