Abstract
Background: Knee osteoarthritis (OA) is well known to be one of the most common joint disease in elderly people and characterized by pain, stiffness and loss of function in articulating joints, which develop slowly and worsen over time. Chondroitin sulfate (CS) and glucosamine hydrochloride are used for the treatment of OA and reported that these substances are favorably modify the clinical symptoms, especially pain, however, the therapeutic mechanisms of these substances are not fully understood. The present study was undertaken to examine the influence of CS on the production of periostin, which is one of the important molecules associated with OA development, by synoviocytes from an OA patient by an in vitro cell culture technique. Methods: Synoviocytes (1 x 105 cell/ml) were stimulated with 10.0 ng/ml IL-13 in the presence of various concentrations of CS. After 48 h, periostin content in culture supernatants was examined by ELISA. We also examined the influence of CS on transcription factor, STAT6, activation and periostin mRNA expression in synoviocytes 12 and 24 h after IL-13 stimulation, respectively. Results: Addition of CS into cell cultures caused the suppression of periostin production from synoviocytes induced by IL-13 stimulation through the inhibition of STAT6 activation and periostin mRNA expression. The minimum concentration of CS that caused significant suppression of periostin production, STAT6 activation and mRNA expression was 10.0 μg/ml. Conclusion: These results strongly suggest that the ability of CS to suppress periostin production from synoviocytes may account, at least in part, for the clinical efficacy of CS on OA.
Highlights
Knee osteoarthritis (OA) is well known to be one of the most common joint diseases and is a major cause of impaired mobility and disability in elderly people
IL-13 was dissolved in Synoviocyte Growth (SG) Medium, sterilized and stored at -40°C until used. mRNA isolation kits (TaqMan Gene Expression Cells-to-CtTM) and real-time reverse transcription-polymerase chain reaction kits (RT-PCR; TaqMan Gene Expression Assays) were purchased from Applied Biosystems (Foster City, CA, USA)
The present results clearly showed that treatment of synoviocytes from an OA patient with Chondroitin sulfate (CS) significantly inhibits periostin production from synoviocytes induced by IL-13 stimulation
Summary
Knee osteoarthritis (OA) is well known to be one of the most common joint diseases and is a major cause of impaired mobility and disability in elderly people. OA is characterized by progressive cartilage erosion mainly caused by the degradation and destruction of proteoglycan and collagen It is observed osteophyte formation and subchondral bone formation in diseased joints [1,4]. In sever OA, the number of periostin positive chondrocytes is increased and the majority of matrix is lost, which is responsible for the development of many deep clefts [1] These reports may suggest that periostin plays crucial roles in pathogenesis of OA. A prominent component of OA guidelines, are used to prevent the development and the persistence of OA [12] These treatments are well known to relieve pain and improve functionally, but not resolve the pathological changes once triggered [12, 14]. The present study, was undertaken to examine the influence of dietary supplements on periostin production from synoviocytes through the choice of an in vitro cell culture technique and CS
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Pharmaceutical Sciences Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
