Abstract
The effect of lanthanum (La) on kainate (KA) responses in neurons acutely dissociated from the rat sacral dorsal commissural nucleus (SDCN) was investigated using the nystatin-perforated patch-recording configuration under voltage-clamp conditions. The responses to KA were mediated by activation of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in SDCN neurons. La<sup>3+</sup> reversibly inhibited KA (100 µM) activated currents (I<sub>KA</sub>) in a concentration-dependent manner over the range from 30 µM to 30 mM, with IC<sub>50</sub> values of 0.64 ± 0.06 mM at a holding potential (V<sub>H</sub>) of –40 mV. Our further study indicated that the effects of La<sup>3+</sup> on I<sub>KA</sub> were voltage independent. Moreover, the inhibition was not use dependent and was not overcome by increasing the concentration of agonist. These findings indicate that La<sup>3+</sup> is an efficacious inhibitor of AMPA receptor mediated responses which may contribute to its cytotoxic effect.
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