Abstract
Ultraviolet (UV)-irradiated adenovirus 2 and 12 suppressed cellular DNA synthesis in growing human embryo kidney cells. The analysis of the mechanism of the suppression revealed that RNA and protein syntheses were not suppressed, thymidine kinase and DNA polymerase were produced normally, cellular DNA was not degraded, and the chain-elongation of nascent DNA proceeded normally. The autoradiograms showed that the suppression was due to the decrease in the number of cells synthesizing DNA, not to the decrease in the rate of DNA synthesis in a nucleus. These observations indicated that the initiation of cellular DNA synthesis was suppressed by UV-irradiated adenovirus. The suppressing activity was found in the UV-irradiated complete virion fraction (density 1.34). Quantitative analyses with purified virions revealed that suppression of cellular DNA synthesis was dependent on multiplicity of infection and 10 3 cell-associated virions suppressed 90-80% of cellular DNA synthesis.
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