Supplementation of multi‐vitamins, not glutamine alleviate erythrocyte oxidative status in rats with trauma‐hemorrhagic shock and resuscitation

Abstract

Resuscitation fluid replacement is the major life saving strategy for patients with hemorrhagic shock; however, resuscitation‐induced oxidative stress may lead to systemic inflammation, multiple organ failure (MOF), and even death. Studies showed that reduction of oxidative stress and preservation of ATP production may improve MOF in animals with trauma‐hemorrhagic shock and resuscitation (THR). In addition, glutamine has been considered as a cellular fuel and is the source of glutathione and multi‐vitamins possess antioxidant activity and play important roles in energy metabolism. Therefore, we investigated the effects of single or simultaneous administration of multi‐vitamins and glutamine on antioxidant defense systems, including enzymatic and non‐enzymatic, in the plasma and erythrocytes of THR. Male SD rats were suffered with THR, including a 5 cm midline laparotomy and mean arterial pressure 30 to 35 mmHg for 60 min followed by resuscitation lactate Ringer's (LR) solution with or without L‐alanyl‐L‐glutamine and/or multi‐vitamins. The sham‐operated, parenterally fed rats were included. After infused with continuous, slow rate of LR solutions for 42 hours, the THR rats had significantly decreased red blood cells and hematocrit and increased erythrocyte glutathione (one‐way ANOVA, p<0.05). Intravenous glutamine significantly increased erythrocyte total and protein‐bound thiol groups and plasma TBARS; intravenous multi‐vitamins significantly decreased erythrocyte TBARS; and combination of glutamine and multi‐vitamins significantly decreased erythrocyte TBARS and the ratio of plasma reduced to oxidized glutathione and increased plasma TBARS. In addition, multi‐vitamins was the main factor to decrease plasma total and protein‐bound thiol groups and plasma and erythrocyte TBARS and to increase the ratio of plasma reduced to oxidized glutathione (two‐way ANOVA, p < 0.05). In conclusion, multi‐vitamins, not glutamine, may alleviate erythrocyte oxidative status in trauma‐hemorrhagic shock and resuscitation.Support or Funding InformationNSC 102‐2320‐B‐030‐005‐MY3This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.