Supplementary Figures 1-5 from A Novel Eg5 Inhibitor (LY2523355) Causes Mitotic Arrest and Apoptosis in Cancer Cells and Shows Potent Antitumor Activity in Xenograft Tumor Models

Abstract

<p>Supplementary Figure 1 (A-C). LY2523355 has potent and selective activity against microtubule-stimulated Eg5 ATPase activity but unlike antitubulins, has no effect on microtubule dynamics in vitro; supplementary Figure 2 (A-E). LY2523355 causes cell cycle arrest at mitosis with monopolar spindle and induces apoptosis; supplementary Figure 3 (A-D). Induction of apoptosis by LY2523355 requires sustained spindle assembly checkpoint (SAC)-mediated mitotic arrest; supplementary Figure 4 (A-D). Antitumor activity of LY2523355 in patient-derived xenograft (PDX) models is correlated with mitotic arrest of cancer cells; supplementary Figure 5 (A,B). Mitotic arrest and apotosis of p388 cells in ascities fluid after continuous infusion of LY2523355, demonstrating the threshold concentration effect.</p>