Abstract
<p>Activation status of T and B cells. (A) T cell gating strategy. T cells from Fig. S1, were split into CD4+ and CD4- (CD8+) T subsets, and subsequently analyzed for the activation markers CD25 and HLA-DR. (B) Percentage of activated CD4+ and CD8 (CD4-) T cell subsets. (C) B cell gating strategy. From B cells, the activation marker HLA-DR (black box) was quantified. (D) Percentage of activated B cells on therapy. Biexponential displaying was done for the dot plots. Mean and {plus minus} SD are provided. B = before treatment; P = on anti-PD-1 therapy; n = number of biopsies analyzed. Solid circles represent responders; open circles represent non-responders (n= 26 baseline; n= 22 on therapy).</p>
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