Supplementary Experimental Procedures, Supplementary Figures 1-7, Supplementary Tables 1-2 from B7-H4(B7x)–Mediated Cross-talk between Glioma-Initiating Cells and Macrophages via the IL6/JAK/STAT3 Pathway Lead to Poor Prognosis in Glioma Patients

Abstract

<p>Supplementary Figure 1. Expression of B7-H4 in GBM from TCGA database. Supplementary Figure 2. WB analysis of GIMs, normal microglia, Model GL261 and Vitro GL261 Supplementary Figure 3. IL-6 and IL-10 expression pattern in serum, CSF, and the glioma microenvironment. Supplementary Figure 4. WB analysis of BV2 microglia stimulated with CD133+ supernatant Supplementary Figure 5. IL-6/Stat3/B7-H4 signal transduction pathway. Supplementary Figure 6. GIMs enriched from CD133+ cells induced inhibition of T cell function via B7-H4 in vitro. Supplementary Figure 7. Outline for the increase of B7-H4 expression by immunosuppressive cross talk between CD133+ cells and GIMs. Supplementary Table 1. Clinical characteristics of the patients for serum, CSF and tissue Supplementary Table 2. Clinical characteristics of the new diagnosed GBM patients for survival analysis</p>