Abstract
Temperature induced superoxide anion radical (O 2 ·−) generation in vivo has been demonstrated in the gills of Heteropneustes fossilis by electron spin resonance (ESR) spin trapping. Temperature exposures from 25°C to 37°C for various times (1–4 hr) caused generation of O 2 ·− in the gill. The acid mucopolysaccharide test was conducted in gill sections during elevated temperatures. The results showed an increased activity of mucopolysaccharide in gills which indicate an increased mucus secretion in gills during elevated temperatures. The detectable stable levels of O 2 ·− in the gill at 32 and 37°C temperature exposures point towards a probable role for this radical in the exudation of mucus under elevated temperature.
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