Abstract
Volatile constituents of black cumin and medlar seeds were obtained by supercritical carbon dioxide extraction and analysed by gas chromatography-mass spectrometry (GC-MS). A total of 16 compounds accounting for 99.36% of the black cumin supercritical fluid extraction (SFE) extracts were identified. γ-terpinene (37.98%), cuminaldehyde (11.48%) and α-methyl-benzenemethanol (25.55%) were the major compounds identified in black cumin. The SFE method has been compared with hydrodistillation. SFE products were found to be markedly different from the corresponding hydrodistilled oil. A remarkable amount of ρ-cymene was present in the hydrodistilled essential oil, also the ratio of γ-terpinene and cuminaldehyde in distilled oil was high in comparison with the supercritical carbon dioxide extract. Also, supercritical fluid extraction of α-methyl-benzenemethanol was more selective. However, cuminyl alcohol was not found in the SFE products. The results showed that by using supercritical carbon dioxide only 3 components represented more than 99.99% of the medlar oil, and benzaldehyde (98.49%) was the major component.
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