Super-hydrophilic and high strength polymeric foam dressings of modified chitosan blends for topical wound delivery of chloramphenicol

Abstract

In the present study polymer blends based on chitosan (CS) and its derivatives with trans-aconitic (t-Acon) acid and another with trimellitic (TRM) anhydride, were prepared for topical wound delivery of chloramphenicol (CHL). FT-IR spectroscopy revealed the successful grafting of t-Acon acid or TRM anhydride into CS macromolecules at molar ratios 1:1 and 1:0.5, while powder X-ray diffraction (XRD) analysis showed that the prepared materials were amorphous. Neat chitosan and its grafted derivatives were mixed in different ratios (25/75, 50/50 and 75/25 w/w) in order to prepare suitable blends. Scanning electron microscopy (SEM) showed that the formed blends after freeze-drying had a sponge-like structure, while thermogravimetric analysis (TGA) verified their thermal stability. All blends are miscible in studied compositions and have extensive swelling and much better mechanical properties than neat CS. In a further step, the obtained porous sponges prepared from CS/CS-derivatives 50/50 w/w were loaded with Chloramphenicol (10, 20 and 30 wt%), a broad-spectrum antibiotic, and the prepared dressings were evaluated in terms of FT-IR, XRD, SEM, and in vitro drug dissolution. An initial burst release followed by a quasi-Fickian diffusion driven sustained release phase was observed while the addition of chloramphenicol gives high antimicrobial properties to all dressings.