Abstract
Coffee husks are abundant lignocellulosic materials that can be used to produce fermentable sugars. This study produced reducing sugars from coffee husks through biological pretreatments using the white-rot fungi Pleurotus ostreatus (PL) combined with steam explosion (160, 180, and 200 °C for 10 min). The results indicate that the biological pretreatment time is three weeks with 10.94% cellulose, 7.15% lignin, and 10.73% hemicellulose biodegraded. The overall yield with PL pretreatment was the 4.19 g reducing sugars/100 g coffee husk, 22% more than the original material. In the combined pretreatment, the maximum overall yield of 26.93 g reducing sugars/100 g coffee husk was achieved using pretreated materials with biological pretreatment for three weeks followed by steam explosion at 200 °C for 10 min. This value was 2.1 times that obtained under the same conditions of steam explosion treatment without fungal pretreatment (13 g reducing sugars/100 g coffee husk). Furthermore, this work demonstrated that combined treatment increased coffee husk sugar production.
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